Background: Infectious diseases impose significant risk to the public health regardless of incredible growth in human medicine. Cow urine is an effective antibacterial substance against a variety of pathogenic microorganism. Hence; the present study was undertaken for assessing the antimicrobial effect of cow urine on Staphylococcus aureusand Streptococcus pyogene. Materials & methods: For the antimicrobial assessment, disease free cow urine was collected. A sterile container was used for collecting free cow urine from local cattle yard. In a sealed glass bottle, fresh cow urine was kept in sunlight for a time period of three days (seventy two hours) before the starting of the experiment, with the aim of making cow urine photo activated. Experimental Staphylococcus aureusand Streptococcus pyogene cultured were obtained from microbiology laboratory. Agar well diffusion method was used for assessing the antibacterial efficacy of cow urine. Also simultaneous pouring of bacterial strains in 2 vials each containing 1 ml of cow urine was done. Assessment of optical density (OD) was done after every one hour. Results: Mean zone of inhibition in relation to Staphylococcus test samples was 21.5 mm while in relation to Streptococcus pyogenes, it was found to be 20.9 mm respectively. The declines in the O.D values indicated that the cow urine is showing its antimicrobial activity. Conclusion: Cow urine possesses remarkable antibacterial activity.
Key words: Antimicrobial, Staphylococcusaureus,Streptococcus pyogenes