Journal of AdvancedMedical and Dental Sciences Research

Aim: Aim of the present studyto determine the seroprevalence of dengue viral infection using IgM antibody capture ELISA for the early diagnosis. Material and methods: This was a prospective observational study conducted in the Department of Microbiology. A total of 600 serum samples from suspected dengue cases attending OPD or admitted in the hospital were tested for the confirmation of Dengue. We have received blood samples in our microbiology laboratory, the blood samples were allowed to clot at room temperature and then we centrifuged the samples and serum samples were separated. From the serum samples we have done NS1 Ag and IgM Ab testing by ELISA. Results: Out Of 600, 100 samples were positive for dengue. Seroprevalence of Dengue was 16.67%. All dengue positive patients in our study had fever of 2 to 7 days. The most common presenting symptoms of dengue were fever with body ache (46%), headache (37%), nausea (34%) and vomiting (23%). Out of 100 dengue cases fever with rash was observed in 7 cases (7%).Out of 100 dengue cases, NS1/NS1+IgM/IgM were positive for 88(88%) patients, suggesting primary infection. IgM and IgG positive was seen in 7(7%) patients, suggesting late primary or early secondary infection. IgG was positive in 5(5%) cases, suggesting secondary or past infection. Conclusion: The present results revealed that the study region is epidemic for dengue viral infection and there is an urgent need for the constant monitoring to control further spreading of the infection in the community, hence serological test have important role in the early diagnosis. Therefore IgM ELISA is recommended in all the suspected dengue patients so as to instigate essential treatment and assessment of morbidity and mortality rate during an outbreak.