Journal of AdvancedMedical and Dental Sciences Research

Objectives: This study aimed : 1.To assess the antimicrobial properties of silver zinc zeolite nanoparticles and Thymoquinone embedded in acrylic denture base resin. 2. To assess the flexural strength of acrylic denture base resin incorporated with silver zinc zeolite nanoparticles and Thymoquinone. 3. To compare the antimicrobial and mechanical properties of acrylic denture base resin incorporated with silver zinc zeolite nanoparticles and Thymoquinone. 4. To assess the surface topography of denture base resin incorporated with silver zinc zeolite nanoparticles and thymoquinone. Materials and methods: Eighty-one acrylic specimens were fabricated and divided into three groups. GROUP A - Acrylic resin (Control group). GROUP B - Acrylic resin containing 1% Silver Zinc Zeolite nanoparticle. GROUP C - Acrylic resin containing 1% Thymoquinone. The acrylic samples were fabricated acrylic specimens, (65 x 10 x 2.5) according to the ADA Specification No.12 for all the three groups. The flexural strength was tested using three-point bending test in a universal testing machine. The test was carried out with a cross head speed of 1mm/min. The results obtained were compared by using One Way Anova Test. For antimicrobial test the acrylic samples were fabricated with dimensions 10× 10 × 3 mm for all the three groups. The specimens were placed in test tube with candida culture. The antimicrobial test was done by using serial dilution test and CFU/ml. Serial dilution was done from 10-2 to 10-5 . The culture was spread on petridish and after 24hrs colonies were counted and CFU/ml was determined. The results obtained were compared by using Kruskal Wallis Test and multiple comparison was done by using Mann Whitney Post hoc Test. The SEM analysis was done for all the three groups. The visual interpretation of the images obtained was done to determine the distribution of antimicrobial material within the polymer matrix. Results: Within the limitations of this study it can be concluded that 1) There was no statistical difference in the flexural strength of all the three. 2) The addition of thymoquinone and silver zinc zeolite nanoparticles significantly decreases the candida albicans count. 3) The addition of thymoquinone in denture base resins showed the least microbial count followed by silver zinc zeolite nanoparticles. 4) There was statistically significant difference of CFU/ml between the control group and other two groups. 5) There was no statistically significant difference of CFU/ml between the acrylic resin specimens incorporated with thymoquinone and acrylic resin specimens incorporated with silver zinc zeolite nanoparticles. 6) The SEM analysis of acrylic specimen incorporated with the thymoquinone showed fair dispersion of material within the polymer matrix and acrylic specimen incorporated with silver zinc zeolite nanoparticles showed little aggregation within the polymer matrix.