Aim: To determine the minimum inhibitory concentration (MIC) of Tulsi extract for Candida. Design and setting: Experimental design, in vitro study, Lab setting. Material and Method: Ethanolic extract of Tulsi was prepared by cold extraction method. Serial dilutions were prepared.(0.5%.1%,2%,3%,4%,5%,6%,7%,8%,9%,10%). Chlorhexidine was used as positive control. These were then subjected to microbiological investigation to check the minimum concentration of the extract which gives wider zone of inhibition. The zones of inhibition were measured in mm using vernier calipers. Result: At 0.5% concentration of Tulsi extract, zone of inhibition of 21 mm was seen. Conclusion: Tulsi extract showed antifungal property and 0.5% concentration of Tulsi is the MIC.
Key words: Antifungal, candida, tulsi extract.
Correspondence to: Dr. Nimesh Jain, Senior Lecturer, Department of Oral Medicine & Radiology, College of Dental Sciences, Rau, Indore, M.P., India
This article may be cited as: Jain N, Jadhav M, Annigeri RG, Pipaliya PR, GK Mangala, J Thimmasetty. Evaluation of Antifungal Activity of Ethanolic Ocimum Sanctum extract. J Adv Med Dent Scie Res 2018;6(9):66-69.