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Volume 6 Issue 8 (August, 2018)

Original Articles

Antimicrobial Effect of Cow Urine and Goat Urine against common Dental Caries Pathogens- An in vitro Comparative Study
Prachi Dave, Vimmie Gill, Pathak Arpita Ajitkumar, Prachi Patel, Anandita Acharya Raghu, Sneha Lakshmi Satya Tayaru Tulluri

Background- The emergence of resistant bacterial strains due to overuse of antibiotics has led to increase in use of natural animal products with known antimicrobial properties as therapeutic agents. Objectives- To assess the antimicrobial potential and Minimum Inhibitory Concentration (MIC) of cow urine and goat urine against common dental caries pathogens. Materials and method:  Distillates prepared from cow urine and goat urine samples were assessed for its antimicrobial activity and minimal inhibitory concentration (MIC) against Streptococcus mutans and Lactobacillus acidophilus using agar well diffusion method. Statistical analysis was performed with One Way Analysis of Variance (ANOVA) using SPSS (Statistical Package for Social Sciences) version 19. Results: Both urine samples showed potent antimicrobial activity with MIC values ranging from 5-10µg/ml against dental caries pathogens. In particular, goat urine sample showed least MIC values of 5µg/ml against S. mutans, L. acidophilus. A statistically significant (p<0.05) zone of inhibition was observed for all samples against S. mutans at highest concentration (50µg/ml). Goat urine resisted the micro-organisms effectively compared to cow urine. Conclusion- Samples of cow urine and goat urine were found to be effective with therapeutic potential against dental caries pathogens. On comparison, goat urine was found to be more potent.
Key words: agar, cow urine, goat urine, Streptococcus mutans, Lactobacillus acidophilus.

 
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