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Volume 4 Issue 5 (September - October, 2016)

Original Articles

A STUDY ON BACTERIOLOGICAL PROFILE OF CONJUNCTIVITIS PATIENTS ATTENDING IN A PERIPHERAL TERTIARY MEDICAL COLLEGE OF WEST BENGAL
Sayantan Mondal, Indrajit Gupta, Ayan Nandi, Gadadhar Mitra

Background: Conjunctivitis is the inflammation of the outermost layer of the white part of the eye and the inner surface of the eyelid. It is the most common cause of a red, irritated eye. The most infectious causes of conjunctivitis are viral followed by bacterial. Bacterial infection tends to produce more mucopurulent discharge. The present study aims at knowing the bacteriological spectrum of conjunctivitis and their antibiogram pattern. Materials and Methods: The present observational descriptive study was conducted in the department of Microbiology and department of Ophthalmology, Burdwan Medical College and Hospital from April 2015 to October 2015. A total of 113 patients were selected from ophthalmology department, having clinical features of suspected conjunctivitis (red eye, itching, foreign body sensation and discharge from eyes.).Sample processing, isolation, and identification of the bacterial isolates were done as per standard microbiological protocol. Antibiotic susceptibility pattern was performed according to CLSI guidelines using antibiotic discs of ofloxacin (5μg), ciprofloxacin (5µg), amoxicillin (20µg), gentamicin (10µg), vancomycin (30µg), gatifloxacin (5µg), tetracycline (30µg), ceftriaxone (30µg), and penicillin (10units) from Hi-media. Results: Out of 113 samples, 54 i.e. 47.69% showed culture positivity. The most common isolated organisms were Coagulase- negative staphylococcus (37.04%) followed by Staphylococcus aureus (29.63%). Other organisms isolated were Streptococcus pneumoniae (5.55%), Pseudomonas aeruginosa (14.81%), Escherichia coli (11.11%), klebsiella pneumonia (1.11%). Analysis of antibiotic susceptibility pattern revealed high degree of resistance prevalent among these isolates. Gatifloxacin (88.89%) and ceftriaxone (81.48%) and vancomycin (94.87%) and gentamicin (86.67%) were found to be most sensitive while tetracycline (73.33%), penicillin (71.80%) showed most resistant to these isolates. Conclusions: Coagulase-negative staphylococci were the commonest isolate from the samples of patients having bacterial conjunctivitis followed by Staphylococcus aureus. Prevalence of gram- positive bacteria was higher than gram negative bacteria. Higher resistance shown by isolates to amoxicillin, penicillin, and tetracycline. Higher prevalence of resistant pathogens indicates injudicious overuse of antibiotics, necessitating implementation of effective antibiotic policy.
Key-words: Conjunctivitis, Gram-positive bacteria, Gram-negative bacteria.

Corresponding author: Dr. Indrajit Gupta, 128/1 G.T.Road, Baidyabati, Kajipara, Dist-Hooghly, West Bengal, Pin-712222., E mail: indrajitgupta.mail@gmail.com

This article may be cited as: Mondal S, Gupta I, Nandi A, Mitra G. A study on bacteriological profile of conjunctivitis patients attending in a peripheral tertiary medical college of West Bengal. J Adv Med Dent Scie Res 2016;4(5):5-10.

 
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