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Volume 4 Issue 4 (July - August, 2016)

Original Articles

ASSESSMENT OF MICROBIAL PROFILE IN THE PATIENTS WITH DIABETIC FOOT- A MICROBIOLOGICAL STUDY
Rajesh K. Yadav, Anil Mishra, Rachna Sharma

Background: Diabetic foot is one of the most feared complications of the diabetes and is the leading cause of the hospitalization among diabetic patients. Neuropathy, peripheral vascular disease, foot ulceration and infection with or without osteomyelitis are few of the several pathological complications leading to the development of gangrene and which even necessi­tates limb amputation. While the foot infections in persons with diabetes are ini­tially treated empirically, a therapy which is directed at the known causative organisms may improve the outcome. Hence; we evaluated the microbiological profile of the patients with diabetic foot. Materials & Methods: A total of 120 patients with diabetes who reported in the hospital  from December 2012 to May 2015, for a period of six months,  were included in this prospective study. The clinical history of the patients such as age, sex, types of diabetes, duration of diabetes, size of ulcer and duration of ulcer were recorded on a proforma. The ulcers were graded according to the Wagner’s grade classification. A total of sixty swabs were collected and processed for bacteriological investigations. The samples were processed by direct inoculation on to culture media like Sheep Blood agar (SBA), Brain Heart infusion Agar (BHIA) and Nutrient Agar (NA) incubated at 37°C for 24 hrs. The bacterial isolates were identified and confirmed according to the Bergey’s manual of Determinative Bacteriology. Results: 54% of the total patients included in the present study were male and rest were female. The mean age group was found to be 59 years. The duration of the ulcer ranged from 10 to 20 years and the enrolled cases were of Wagner’s grade I to III. In our study, approximately 70% of patients were Grade I. Although in gram positive organism Staphylococcus aureus was the predominant isolate followed by Staphylococcus saprophyticus, while Pseudomonas aeruginosa was the predominant isolate followed by Escherichia coli in gram negative organism. Conclusion: Diabetic foot is one of the common infections with prevalence among diabetic patients. Also the gram negative infection was seen in higher quantity in diabetic foot patients in the present study.
Key Words: Diabetic foot, Microbiology

Corresponding Author:  Dr. Rajesh K. Yadav, Associate Professor, Department of Microbiology, T.S.M.M.C. &H. Amausi,  Luknow, Uttar Pradesh, India

This article may be cited as: Yadav RK, Mishra A, Sharma R. Assessment of microbial profile in the patients with diabetic foot: A Microbiological Study. J Adv Med Dent Scie Res 2016;4(4):105-108. 

 
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