Journal of AdvancedMedical and Dental Sciences Research

Introduction: The process of digesting germs or foreign particles is carried out by macrophages. There are various macrophages according to location of the macrophages, such as; alveolar macrophages (dust cell), liver macrophages (kupffer cell), skin macrophages (Langerhans cell), bone macrophages (osteoclast cell), peritoneal macrophages and others. Macrophages are specialized phagocytes that play an important role in against foreign invasion. The purpose of this study is to determine whether peritoneal macrophages that have been stained by neutral red, are they still able to do phagocytosis again. Method: This study was experimental laboratory research which was performed in two group, the first group was control group. The second group was those given neutral red injection, and each rat put 4 plastics into his abdomen. The rats were sacrificed after treatment, and made histological slide with Giemsa staining for peritoneal macrophages observation. The data was analyzed by using Cochran analyzed. Result and Discussion: The results show that were six histological slide, and each slide is examined on six views. Then there were 48 peritoneal macrophage descriptions were observed. The observation turned out to be 95.83% there were peritoneal macrophages that have bubbles and attached to the plastic, whereas in the rat control no bubbles were found. Conclusion: This study suggested that there was a relationship between twice phagocytic with peritoneal macrophages which contain neutral red, then attached to the plastic. Key words: peritoneal macrophages, neutral red, phagocytosis, plastic.