Journal of AdvancedMedical and Dental Sciences Research

Background: Diabetes mellitus is a metabolic disorder characterized by hyperglycemia along with biochemical alterations of glucose and lipid peroxidation. It produces free radicals that induce lipid peroxidation which acts as an indicator for oxidative stress in the body. The aim of the present study was to evaluate the total antioxidant status in relation to oxidative stress in Type 2 Diabetes Mellitus. Material and methods: The present study was case control study conducted among a population of 110 Type 2 diabetic patients in the age group of 30- 60 years with the similar number of age and sex matched healthy controls. 3ml venous blood was drawn in the fasting condition from the patients. Two hours after food 1ml of venous blood sample was collected into tube with oxalate-fluoride mixture for Post Prandial Plasma Sugar (PPPS) estimation. FPS & PPPS were estimated using Glucose Oxidase Peroxidase (GOD POD) Method (Enzymatic method). Colorimetric assay with Cayman kit Cayman’s antioxidant assay Kit was used to measure the total antioxidant capacity of plasma, in the control and cases. Results: The Fasting Plasma Sugar values in diabetic subjects were 172.43 ± 43.02 mg/dl compared to the healthy controls 89.52 ± 10.21 mg/dl). The Post Prandial Plasma Sugar values among diabetic subjects was 247.26 ± 46.16 mg/dl) and in controls was 115.34±42.18 mg/dl). There is significant increase in MDA levels among Diabetic patients 4.01±0.78μM in comparison to the controls (1.99±1.22 μM). In our study, there has been decreased total antioxidant status among diabetic cases as 0.49±0.42 mM whereas the healthy controls had a value of 1.73±1.41 mM. Conclusion: Our study concluded that in Type 2 DM MDA levels were increased and total antioxidant status was decreased. Key words: Type 2 Diabetes mellitus, total antioxidant status, Oxidative Stress.