Aim: The aim of the study was to analyze the immunoexpression of p53 and Bax and to investigate the relationship of p53andBax in Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (Oral SCC). Material and Methods: This study was performed on histopathologically confirmed samples of 90 OED (30 mild, 30 moderate and 30 severe dysplasia), 90 samples of Oral SCC (30 well differentiated, 30 moderately differentiated and 30 poorly differentiated SCC) and 30 oral epithelium for p53 and Bax protein expressions by immunohistochemical (IHC) method. The data were analyzed using chi-square and Kruskal-Wallis tests. Result: The quantification of IHC study result revealed that the expression of p53protein was confined to the basal layer but, Bax was expressed in both basal and suprabasal layers in oral epithelium. Whereas, p53 and Bax were expressed in basal, suprabasal layers in OED and Oral SCC. The expression of p53 and Bax was 16.7% and 56.7% in oral epithelium respectively. The p53 expression in mild, moderate and severe dysplasia was 76.7%, 83.3% and 93.3% respectively, the Bax expression was 86.7%, 60% and 50%.The p53 expression in well, moderate and poorly differentiated Oral SCC was 90%, 60% and 40% respectively,Bax expression was 73.3%,40% and 26.7%. The comparison between oral epithelium, OED and Oral SCC showed statistically significant for p53 (p-0.000) and Bax(p-0.028). The association between p53 and Bax expression was not significant in oral epithelium (p-0.568) and OED (p-0.174) while, it was significant in Oral SCC (p-0.000). Conclusion: The immunoexpression of p53 and Bax showed significant among oral epithelium, OED and Oral SCC. The significant association of p53 and Bax in Oral SCC suggested that the influence of p53 on Bax induced apoptosis probably played a role in malignancy.
Key words: Oral epithelial dysplasia, oral squamous cell carcinoma, pro apoptotic protein, tumor suppressor.
Received: 15 December 2018 Revised: 27 December 2018 Accepted: 28 December 2018
Corresponding author: Dr. Sabitha S, 19-3D; ARRS Apartment, Greenways road, Fairlands, Salem 636016. Tamilnadu. India
This article may be cited as: S Sabitha, Mohan J, Nirmal M, P Vasanthan, JS Sathiya. Association of p53 and Bax in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma – An Immunohistochemical study. J Adv Med Dent Scie Res 2019;7(1):67-71.