Journal of AdvancedMedical and Dental Sciences Research

Aim: The purpose of this research is to investigate the efficiency of a modified Hodge test in detecting carbapenemase production in Klebsiella pneumonia in an in vitro setting. Materials and Methods: It was determined to be necessary to collect a total of 250 Gram-negative rods from a range of clinical samples. Based on the disc diffusion results, the researchers only included in the study those isolates that demonstrated intermediate or sensitive zones between 16 and 21 millimeters in diameter. The next step was to perform the Modified Hodge test on the samples that were previously isolated. Results: The results of the research revealed that out of a total of 250 isolates, 50 of them tested positive for the production of carbapenemase when the test was conducted using the Modified Hodge method. Twenty percent of individuals who were diagnosed with Klebsiella pneumonia also developed carbapenemase in their bodies. Conclusion: The modified Hodge test is a straightforward procedure that can be carried out in a standard laboratory setting in order to identify carbapenemases in clinical isolates that exhibit either an intermediate or sensitive zone diameter on disc diffusion. This is done for the purpose of determining whether or not the carbapenemase in question is a carbapenemase. It is of the utmost importance that any and all isolates that exhibit an intermediate or sensitive zone diameter on disc diffusion be tested for the production of carbapenemases using a modified version of the Hodge test, and that these results be further verified by PCR. This is because it is of the utmost importance that any and all isolates that exhibit an intermediate or sensitive zone diameter on disc diffusion.