Journal of AdvancedMedical and Dental Sciences Research

Aims and objectives: The aim of the present to evaluate the incidence and associated comorbidities which influence the surgical site wound infection. Material and methods: The study was a cross sectional study which was carried in the Department of microbiology. Using sterile cotton swabs, two pus swabs/ wound swabs were collected aseptically from each patient suspected of having SSI. Gram stained preparations were made from one swab for provisional diagnosis. The other swab was inoculated on nutrient agar, 5% sheep blood agar (BA) and MacConkey agar (MA) plates and incubated at 37°C for 24-48 hours before being reported as sterile. Growth on culture plates was identified by its colony characters and the battery of standard biochemical tests. All the isolates were tested for antimicrobial susceptibility by Kirby Bauer disk diffusion technique on Muller Hinton Agar. Results: Out of 250 samples, 100 samples were culture positive (40%). Among 100 positive samples 55(55%) were males. The age wise distribution of the gender has been shown in the with maximum no. of culture positive samples in age 20-30 years (32%) followed by 30-40 (17 %) and then followed by 40-50 (16%) of age group respectively. Out of 100 cultures positive samples Escherichia coli. 27(27%) was the most common pathogen isolated followed by S.aureus 25 (25%), Citrobacter spp. 16(16%) and Klebsiella spp 9(9%), Pseudomonas aeruginosa 8(8%), Enterobacter spp. 6(6%), CONS 6(6%), Acinetobacter spp 2(2%) and Proteus spp. 2(2%) respectively. Conclusion: It has been concluded that wound infections in this were polymicrobic in nature and, in most cases, associated with S. aureus, E.coli and Pseudomonas aeruginosa.