Journal of AdvancedMedical and Dental Sciences Research

Background: Prostate cancer is the second most frequently diagnosed cancer as well as the sixth leading cause of death in males with increasing incidence worldwide. The present study was conducted to assess role of P501S (prostein) in the diagnosis of prostate carcinoma. Materials & Methods: 58 prostate samples were fixed in buffered formalin, followed by paraffin embedding and stained with hematoxylin and eosin (H and E). Immunohistochemistry (IHC) was performed on Ventana automated stainer. The intensity of positivity was scored from 0 to 3 as follows: score 0 = nonstained; score 1 = weak; score 2 = moderate; and score 3 = strong. Results: Diagnosis found to be normal epithelium in 8, benign prostate hyperplasia (BPH) in 15, primary adenocarcinoma in 22, metastatic adenocarcinoma in 7 and HGPIN in 6. The difference was significant (P< 0.05). Protein expression and intensity score for normal epithelium was 100% and 1-8-2, in BPH was 100% and 2-2-7, in primary adenocarcinoma was 100% and 2-2-4, in metastatic adenocarcinoma was 100% and 1-2-2 and in HGPIN was 100% and 2-2-2 respectively. Conclusion: Prostein showed a specificity of 100% in differentiating various prostatic carcinoma hence, can be used in prostate lesions. Key words: Adenocarcinoma, Prostein, Prostate.