Journal of AdvancedMedical and Dental Sciences Research

Background: The universal drug sensitivity testing (UDST) approach can lead to major changes in pattern of detection of resistance among TB patient. The resistance that develops in a patient who has received prior chemotherapy is defined as acquired drug resistance. This change needs to be assessed and addressed earliest so that any deficiency or weakness if found can be assessed and changes in strategy can be done within time frame. The aim of this study evaluated the various factors associated with drug resistance tuberculosis among presumptive drug resistant tuberculosis patients. Material & Methods: A hospital based prospective observational study done on 100 cases done in the Department of Respiratory Medicine, SP Medical College, Bikaner. Sputum for acid fast bacilli, smear microscopy, culture and drug susceptibility tests were performed at Department of Microbiology, SP Medical College, Bikaner. Drug susceptibility testing of the samples was performed by the radiorespirometric Buddemeyer technique (a manual modification of the Bactec 460 technique). The value of the mean Difference in growth indices (ΔGI) in the triplicate drug containing vials was compared to that for 1:100 control for the same day. If ΔGI was less in the drug containing vials than the 1:100 control, the bacteria were considered susceptible; if more, they were considered resistant. Results: Our study observed that out of 100 cases, 85 (85%) cases had drug sensitive of TB & 15 (15%) cases had drug resistant of TB. Male preponderance were occurred in drug sensitive (66%) and drug resistant TB (11%). Among 15 drug resistant TB cases, 12 cases were resides in rural areas and 3 cases were reside in urban areas. Sputum positivity status of DR-TB patients, mostly DR-TB cases (40%) had 3+ followed by 2+ in 26.66% cases, 1+ in 13.33% cases, negative in 13.33% cases and only 6.66% cases had scanty in agar plate (table 2). The mostly DR-TB occurred in previously treated case (80%) and 20% cases had newly diagnosed cases of TB. Conclusion: We can easily conclude from the present study that well administered and dedicated first line treatment for susceptible cases is the need of hour to prevent development of resistance in such cases. Keywords: TB, Drug sensitive, Drug resistant, Sputum positive