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Volume 7 Issue 9 (September, 2019)

Original Articles

Assessment of HPV 16/18 in oral precancerous lesions
Spinder Kour, Adil Rasool Malik

Background: Cancer has afflicted man throughout recorded history. The role of saliva as a diagnostic tool has advanced exponentially over the past decade. Human papilloma virus (HPV) is a small, epitheliotropic, nonenveloped DNA virus. Hence; under the light of above mentioned data, the present study was undertaken for assessing the presence of human papillomavirus (HPV) 16/18 in saliva rinses of patient with oral precancerous lesions. Materials & methods: A total of 40 subjects were enrolled in the present study. Among these 40 patients, 20 were healthy controls while the remaining 20 were patients with oral pre-cancerous lesions. Saliva of oral precancerous lesion patients and healthy controls sample was collected by 10ml normal saline rinses that was gargled and expectorated. The sample was then labeled, placed in the provided specimen transport bag, and sent to the laboratory for Detection of HPV in saliva by Polymerise Chain Reaction. Results: HPV 16/18 positivity was found to be present in 2 cases of the pre-cancer group while HPV 16/18 negativity was found to be present in 18 cases of the precancer group. HPV 16/18 activity was found to be negative in all the cases of normal controls. Conclusion: No significant difference was observed while comparing the prevalence of HPV 16/ 18 in patients with pre-cancer lesion. Key words: Human papilloma virus, Pre-cancer lesion

 
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