Categories

Volume 3 Issue 1 (January - March, 2015)

Original Articles

EVALUATION OF MECHANICAL EFFICACY OF WAVE-ONE-TM AND F2 PROTAPER-TM USING RECIPROCATING MOTION AND PROTAPER-TM SYSTEM UP TO F2 USING ROTARY MOTION IN REDUCING E. FAECALIS COUNT
Shachi Mehta, Kiran P. Singbal, Anisha Merh, Roshni Rai

Background: To evaluate the mechanical reduction of the bacterial population in the root canal using conventional full sequence ProTaperTM universal system in rotary motion, single- file F2 ProTaperTM and Wave-OneTM single file in reciprocating motion. Methodology: 46 extracted human lower bicuspids with a single root canal were selected for the study. Conventional access preparations were made and root canals instrumented 1 mm beyond the apical foramen with K-type files up to size 20. To make both handling and identification easier, the teeth were then mounted vertically in plaster blocks, which were then packaged and sterilized in an autoclave for 20min at 121˚c. Teeth were inoculated with pure culture of E.Faecalis grown anaerobically in brain heart infusion broth. The blocks were placed in self-sealing pouches and incubated at 37°C for 24h. Then 4 samples out of 46 served as controls (2 negative and 2 positive control) and remaining 42 teeth were divided into 3 groups. Group-1 instrumented with conventional full sequence of ProTaperTM universal system in rotary motion, Groups-2 instrumented with single-file F2 ProTaperTM in reciprocating motion and Group-3 prepared with Wave-OneTM single file in reciprocating motion. All root canals were sampled before and after instrumentation. Canals were filled with sterile 0.85% saline solution and each sample taken by using three paper points. All procedures were performed by one operator. Paper points used to sample the canals were transferred to eppendorf tubes containing 1 ml of 0.85% saline solution and vortexed for 1 min. After 10-fold serial dilutions in saline, aliquots of 0.1 ml were plated onto Brain Heart Infusion agar and incubated at 37°C for 48 h. The colony forming units grown were counted. Data obtained from samples taken before and after instrumentation was converted to log and analyzed statistically for differences. Results: Conventional full sequence ProTaperTM universal system showed significantly more bacterial reduction as compared to single file F2 ProTaper TM and Wave-OneTM in reciprocating motion. Conclusion: Conventional full sequence ProTaperTM Universal system used in rotary motion showed more bacterial reduction as compared to Single-file F2 ProTaperTM and Wave-OneTM used in reciprocating motion.

 
Html View | Download PDF | Current Issue