Objective: Oral cancer is one of the major causes of death all over the world. Diagnosing it in the earlier stages is important. Here a cytomorphometric evaluation is used to obtain information on cellular and nuclear events. The objective of the present study is to determine the variation in cellular area, nuclear area, cellular diameter, nuclear diameter and their ratios (NCA/NCD) respectively in healthy individuals, Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma patients. Methods: Exfoliated buccal mucosal cells collected from study subjects (n = 90) from three groups and stained using rapid Papanicolaou stain. Photomicrograph of 50 non overlapping cells captured at 40× magnification with a digital opt scopes microscope image analysis software. Image analysis was performed to obtain cellular diameter (CD), cellular area (CA), nuclear diameter (ND), nuclear area (NA), nuclear to cellular area (NCA) and nuclear to cellular diameter (NCD). These values were statistically compared among the groups using one-way analysis of variance (ANOVA) and Unpaired t test. Results: Study showed a significant reduction in the cellular diameter and cellular area. Increase in the nuclear diameter and nuclear area and their ratios in second and third group. Conclusion: Cytomorphometric changes could be the earliest indicators of cellular and nuclear alterations and could serve as a useful adjunct in the early diagnosis of premalignant lesions and conditions.
Key words: Oral Squamous Cell Carcinoma, Oral Submucous Fibrosis, Exfoliative Cytology, Rapid Papanicolaou.
Received: 2 December 2018 Revised: 27 December 2018 Accepted: 28 December 2018
Corresponding Author: Dr. Arora A, Post Graduate, Dept of Oral & Maxillofacial Pathology & Oral Microbiology, SDC, Meerut, Uttar Pradesh, India
This article may be cited as: Arora A, Reddy V, Verma S. Retrospective Cytomorphometric Analysis of Buccal Squames in Oral Squamous Cell Carcinoma, Different Stages of Oral Submucous Fibrosis and its Comparison to Healthy Individuals. J Adv Med Dent Scie Res 2019;7(1):125-133.