Background: Oral cancer is the fifth common cancer in the world, culminating in over 600,000 new patients annually.Presence of HPV- 16 and 18 in malignant lesions suggests its importance as high risk factor for oral carcinogenesis. The aim of our study was to evaluate the presence of human papillomavirus (HPV) 16/18 in saliva rinses of patient with oral precancerous lesions and oral cancer and to analyse the possibility of using saliva as a diagnostic tool for screening high-risk patients. Materials & methods: Group I Pre cancer Group: This group included 20 saliva samples of clinically and histopathologically diagnosed cases of Oral precancerous lesions. Group II Cancer Group: This group included 20 saliva samples of clinically and histopathologically diagnosed cases of Oral cancer. Group III Normal Group: This group included 20 saliva samples of normal healthy patients, age and sex matched controls. Saliva of oral precancerous and cancerous lesion patients sample was collected by 10ml normal saline rinses that was gargled and expectorated. The funnel was removed; the collection tube is sealed with a cap. All the samples were sent to laboratory for further testing. DNA Extraction was using commercial kit from QIAGEN and PCR. Results: In the cancer group out of the 20 patients 1 was found to be positive for HPV 16 DNA.(5%). In the precancer group out of the 20 patients1 was found to be positive for HPV 16 DNA.(5%). In the control group out of the 20 patients 0 were found to be positive for HPV 16 DNA. (0%). In the cancer ,precancer and control group all the samples were found to be negative for HPV 18 DNA. The distribution of No. of copies of Viral DNA / Genome / Cell did not differ significantly between two study groups.P-0.667 (Non-Significant). Conclusion: In terms of HPV therapeutics, a major focus of the research community could be done in targeting E6 and E7.
Key words: HPV, leukoplakia, oral cancer.
Received: 2 November 2018 Revised: 24 December 2018 Accepted: 28 December 2018
Corresponding Author: Dr. Girija Kunjir, Address: Flat no. 18, Siddhartha Apartments, Gaikwadnagar, Aundh, Pune Maharashtra- 411007
This article may be cited as: Darawade AD, Kunjir GU, Raut AS, Mudgade DK, Kalyanpur KK, Phad UN. Detection of Human Papilloma Virus in Saliva of Oral Precancerous and Cancerous Patients. J Adv Med Dent Scie Res 2019;7(1):117-120.