Background: Growth plays major rolein treatment planning and retention in orthodontics and it has been under scrutiny for years. This research introduces a new method of growth prediction with serum Insulin- like growth factor- 1 and its correlation with established methods of growth prediction. Method: 150 subjects were divided into 5 groups according to 5 stage CVMI stages. 1ml of serum was obtained from 2.5 ml of blood sample collected from each subject using minimally invasive technique. The blood was centrifuged at 3000 rpm for 5 minutes. After centrifuging the serum was separated and stored in different vials. The serum IGF-1 levels determination was done using IGF-1 600 ELISA kit (96 wells) using ELISA method.Results: IGF-1 levels were seen to start low in the prepubertal stages (CVMI 1 and MP3 - F) with a sharp increase from CS2 and MP3- FG to the peak levels at CS3 and MP3 - G. Between CS4 and CS5 and MP3 - H to I, IGF-1 level gradually declined. The values were specific for each stage, denoting that they can be used to differentiate one stage from another. Conclusions: Statistically significant correlation was observed on comparing all the 3 variables. Hence, IGF-1 can be used as a reliable method in predicting growth potential.
Key words: Insulin Like Growth Factor, Skeletal Maturity.
Received: 2 November 2018 Revised: 27 December 2018 Accepted: 28 December 2018
Corresponding Author: Dr. Sweta Bhattacharya, Post graduate student, Department of Orthodontics and Dentofacial Orthopedics, DPU Vidyapeeth, Pimpri, Pune, Maharashtra, India
This article may be cited as: Dhoka SR, Deshmukh S, Jethe S, Rahalkar JS, Bhattacharya S. Reliability of Biological Marker, Insulin Like Growth Factor-1 (IGF-1) as an Indicator in Assessing Skeletal Maturity Using Blood Sample By ELISA Technique. J Adv Med Dent Scie Res 2019;7(1):104-112.