Background: Early administration of adequate antibiotic therapy has been shown to reduce mortality associated with bloodstream infection and has a positive impact on the outcome of bacteraemic patients. Septic shock is defined as sepsis associated with evidence of organ hypoperfusion and a systolic blood pressure <90 or >30 mm Hg less than the baseline or a requirement for the use of vasopressor to maintain the blood pressure. About 70% of Staphylococci isolated in blood cultures are resistant to Penicillin, the most effective antibiotic against the Staphylococcal isolate is Clindamycin (70%) followed by Vancomycin (40%). The aim of present study is to establish the antibiotic sensitivity pattern amongst various blood culture isolates obtained from neonatal blood cultures. Materials and methods: Department of microbiology and department of paediatrics of Integral Institute of Medical Sciences conducted a hospital based study involving 80 children and neonates.The ethical committee clearance was obtained prior to initiating the study and parents of all the subjects were informed about the study and a written consent was obtained from all.By using a sterile syringe blood was drawn through venipuncture before administration of antibiotics. Blood cultures were done by Bactec blood culture technique. Smears were prepared from positive blood cultures and examined. After cultures, bacteria were obtained in pure subcultures. The organism was isolated in pure culture on a solid medium. Isolated colonies was inoculated in a suitable broth medium and incubated at 35-370C for 4-6 hours. The density of the organism in broth was adjusted to approximately106 cfu/ml by comparing it’s turbidity with 0.5 McFarland opacity standard tubes. The antimicrobial susceptibility testing was done by Kirby-Bauer’s Disk Diffusion. The data thus obtained was arranged in a tabulated form. It was expressed as percentage of the total. Results: Out of the total of 80 newborns and children, 40% were females and 60% were males. S.aureus were more sensitive to neticllin, vancomycin, teicoplanin, linezolid & tigecycline(100%) followed by amikacin & tobramycin(93.75%). Coagulase negative Staphyolococci was found to be highly sensitive to vancomycin (100%), teicoplanin (100%), linezolid, netillin, tobramycin, tetracycline & tegicycline (100%) followed by clindamicin 80% and complete resistance (100 %) was seen with penicillin while 80% was seen in cefoxitin, amoxyclav, ciprofloxacin & ofloxacin. Candida albicans shows maximum sensitivity pattern to voriconazole (100%), itraconazole, fluconazole (85.71%) followed by amphotercin –B (57.14%). Conclusion: Staphylococcus aureus was found to be highly sensitive to vancomycin, teicoplanin, linezolid, netillin & tegicycline. Enterococcus was highly sensitive to tetracycline.E.coli was more sensitive to piperacillin/tazobactam, tegicycline and imipenem/cilastin. Candida albicans shows maximum sensitivity pattern to voriconazole.
Keywords: Antibiotic, bactec, staphyloccus, venipuncture.
Corresponding author: Dr. Abhishek Jaiswal, Tutor, Department of Microbiology, TSM Medical College and Hospital Lucknow, U.P. India
This article may be cited as: Jaiswal A, Mishra A Yadav RK, Agarwal L. Antibiotic suspectibility pattern of various bacterial and fungal species isolated from blood culture of children- A prospective study. J Adv Med Dent Scie Res 2017;5(3):76-83.