Categories

Volume 4 Issue 5 (September - October, 2016)

Original Articles

ASSESSMENT OF VARIOUS LABORATORY DIAGNOSTIC METHODS FOR CUTANEOUS TUBERCULOSIS- A RETROSPECTIVE ANALYSIS
Poonam Singh, Manisha Bhargava

Background: One of the challenges in the field of diagnostic pathology is the search for bacilli in a cutaneous tuberculosis (CTB) lesion are a challenge. For increasing the accuracy of diagnosis of cases of latent and CTB, several laboratory diagnostic methods have been revised with times which have higher sensitivity and specificity. Hence; we evaluated the laboratory results of the skin biopsy specimens, in which final diagnosis of CTB was confirmed. Materials & Methods: The present study included 22 skin biopsy specimens which were submitted to the department of general pathology from 2011 to 2014 and were processed with AFB staining, growth cultural in mycobacterium cultural medium and PCR evaluation. PCR technique was used for the assessment sequence and mutations in the core region that are associated with rifampicin resistance. All the results were analyzed by SPSS software. Results: 9 out of 22 patients were positive as CTB by the recovery of M. tuberculosis in culture and/or histopathological affirmation. All the 9 patients showed a negative staining pattern for AFB staining. Five out of nine patients showed positive cultural growth characteristics.  All the patients showed positive CTB findings compatible with the diagnosis of CTB. Only three patients out of 9 showed positive PCR technique. Tuberculosis verrucosa cutis was the most common biological variant detected. Conclusion: A combination of cultural, staining and PCR techniques should be used for increasing the specificity and sensitivity of the diagnostic methods for the diagnosis of CTB.
Key words: Cutaneous, Mycobacterium, Tuberculosis

Corresponding Author: Dr. Poonam Singh, Assistant Professor, Career Institute of Medical Sciences Lucknow, U.P

This article may be cited as: Singh P, Bhargava M. Assessment of various laboratory diagnostic methods for cutaneous tuberculosis: A retrospective analysis. J Adv Med Dent Scie Res 2016;4(5):11-13.

 
Abstract View | Download PDF | Current Issue