Introduction: In forensic studies different methods are utilized for gender determination. Generally canine tooth is utilized for gender determination because they are the most stable teeth, they bear greatest amount of sexual dimorphism and are also resistant to disease and post mortem insults. Aim: Gender determination using mandibular canine index in north-east population living in Paonta Sahib (Himachal Pradesh) and to assess its reliability. Material and methods: The study population comprised 100 subjects that are 50 males and 50 females with an age range of 18–30 years. Measurements were made in millimetres at the contact point of mesiodistal width of the right and left canines and intercanine distance intraorally. The mandibular canine index (MCI) was calculated. Results: There was a significant difference observed between right and left canine width, right mandibular canine index and intercanine distance between males and females. The overall accuracy for gender determination in our study was found to be 65.7%. Statistical Analysis Used: Independent sample t-test was used. P < 0.05 was considered statistically significant. Conclusion: It is evident from our study that the mandibular canine index is a valuable tool for gender determination.