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Volume 7 Issue 7 (July, 2019)

Original Articles

A Comparative Study to Estimate levels of salivary catalase, alpha- amylase and cotinine levels in levels in chronic smokers and non smokers
Krishna Singh Arora

Background: Smoking is considered a health problem at present all over the world.  Smoking is known to have potential effect on body’s immune system, antioxidants level and salivary cotinine levels. Hence; the present study was planned to estimate levels of salivary catalase, alpha- amylase and cotinine levels in levels in chronic smokers and non smokers. Materials & Methods: The present cross-sectional study included assessment of salivary parameters of smokers and non- smokers. A total of 200 subjects were analysed out of which 100 were active smokers and 100 were non- smokers. Unstimulated salivary samples were taken and assessment of alpha- amylase levels was done using biochemical kit and spectrophotometer. For assessment of salivary catalase activity was done using Luck method. For the determination of cotinine levels, Bioassay Technology Laboratory kit was used using ELISA technique.  Data obtained was statistically analyzed. Results: Levels of α- Amylase in smokers and non- smokers group was 204.45 and 165.05 U/ ml respectively. While comparing the salivary α- Amylase levels among the two study groups, we observed non- significant results (P- value > 0.05).   Salivary catalase levels in the smokers group and in the non- smokers group was found to be 6.25 and 10.12 U/ ml respectively. Non- significant results were obtained while comparing salivary catalase levels among the smokers and non- smokers group (P- value > 0.05). Values of salivary cotinine levels among smokers and non- smokers group was found to be 16.20 and 0.76 pg/ ml respectively. We observed statistically significant results while comparing mean cotinine levels among smokers group and non- smokers group. Conclusion: Based on the results of the present study, salivary cotinine levels were higher in smokers than non- smokers.

Key words: α- Amylase, Catalase, Cotinine, Smoking

Received: 13 April, 2019                            Revised:  10 June 2019                             Accepted: 11 June 2019

Corresponding author: Dr. Krishna Singh Arora, Department of Oral Pathology and Microbiology, Genesis Institute of Dental Sciences & Research, Ferozepur, Punjab
                                                                                 
This article may be cited as: Arora KS. A Comparative Study to Estimate levels of salivary catalase, alpha- amylase and cotinine levels in levels in chronic smokers and non smokers. J Adv Med Dent Scie Res 2019;7(7): 82-85.

 
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