Journal of AdvancedMedical and Dental Sciences Research

Background: Periodontitis results from the inflammatory response of the host to the bacterial challenge in the gingival crevicular area. Regulated on activation, normal T cell expressed and secreted (RANTES) is a member of the C-C chemokine subfamily and is produced by fibroblasts, lymphoid and epithelial cells of the mucosa. Hence; the present study was undertaken for assessing the RANTES levels in GCF of patients with periodontitis. Materials & methods: A total of 25 patients with radiographic and clinical diagnosis of periodontitis were included in Group 1 and 25 healthy subjects were enrolled as Group 2. For collection of GCF, area was isolated with cotton rolls and crevicular area was dried using an air syringe. GCF was collected by placing the filter paper strips into the sulcus for thirty seconds. Obtained samples were transported to the laboratory in sterile test tube where ELISA technique and auto-analyzer was used for evaluation of RANTES levels. All the results were recorded and analyzed by SPSS software. Results: Mean RANTES levels of the patients of the periodontitis group and the control group was found to be 69.4 pg/µL and 13.4 pg/µL respectively. While comparing the mean RANTES levels of the patients of the periodontitis group and the control group, significant results were obtained. Conclusion: RANTES levels are significantly raised in periodontitis patients highlighting their role in the pathogenesis of the disease. Key words: Periodontitis, RANTES