Journal of AdvancedMedical and Dental Sciences Research

Background: Detection of morphological and nuclear abnormalities of the cell are the basis of cytological evaluation and for that, micronucleus frequency evaluation in buccal exfoliative cells is a preferable method for cancer risk assessment because it is less technique sensitive, easy and an appreciable biomarker for genomic instability. Hence; under the light of above mentioned data, the present study was undertaken for evaluating micronuclei frequency in exfoliated buccal mucosal cells in Type-2 diabetes patients with tobacco chewing habit. Materials & methods: A total of 75 subjects were enrolled and were divided into three study groups as follows: Group A: 25 Diabetic with tobacco chewing habit, Group B: 25 Non-diabetic with tobacco chewing habit, and Group C: 25 Controls. The cyto smears was separately stained with PAP and GIEMSA stains. The slides were mounted with cover glass using DPX mountant and were analysed. Average frequency of MN= Total no of MN/ Total no of cells with MN. Recording of all the results was done in Microsoft excel sheet and were analysed by SPSS software. Chi- square test was used for evaluation of level of significance. Results: Mean MF frequency per high power field by GIEMSA stain among subjects of group A, Group B and Group C was found to be 1.19, 0.98 and 0.69 respectively. Significant results were obtained while comparing the mean MN frequency per high power field in GIEMSA stain and PAP stain. Conclusion: Type-2 diabetic patients with habits of tobacco smoking had significantly higher frequency of Micronuclei as compared to patients with diabetes and control group. Key words: Buccal mucosa, Exfoliated, Diabetes