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Volume 7 Issue 7 (July, 2019)

Original Articles

Antioxidant status in type II diabetics and non diabetics- A biochemical study
Zhahid Hassan, Afreen Nadaf, Sonia Gupta

Introduction: Diabetes mellitus is a heterogeneous group of metabolic disease showing features of hyperglycemia which results from disorders in carbohydrate, fat and protein metabolism. Free radicals are produced which induce lipid peroxidation which acts as an indicator for oxidative stress in the body. Defensive system in the body consists of antioxidant enzymes like reduced glutathione (GSH) and superoxide dismutase (SOD) which help in scavenging free radicals. Aims and objective: To estimate and correlate the levels of GSH and SOD in saliva and serum of both diabetics and non-diabetics individuals. Materials and method: Unstimulated saliva and venous blood samples were obtained after 12 hoursofovernight fast and the samples were transported to the laboratory. Supernatants of the centrifuged samples were used for the determination of GSH and SOD. Results: The antioxidant enzymes i.e. GSH revealed a statistically significant decrease in their values among the diabetic groups. The levels of SOD in the serum and saliva of diabetic individuals indicated a significant increase in their values when compared with the serum and saliva of control individuals.There was a strong significant positive correlation between serum GSH and salivary GSH in diabetic as well as in control group. Conclusion: Saliva and serum can be used as a diagnostic tool for the estimation of GSH and SOD.
Key words: Diabetes mellitus, antioxidant enzymes, saliva, serum, reduced glutathione

Received: 13 March, 2019                                         Revised:  10 April 2019                             Accepted: 12 April 2019

Corresponding author: Dr. Zhahid Hassan
               
This article may be cited as: Hassan Z, Nadaf A, Gupta S. Antioxidant status in type II diabetics and non diabetics: A biochemical study. J Adv Med Dent Scie Res 2019;7(7): 196-198.

 
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