Journal of AdvancedMedical and Dental Sciences Research

Background: Human oral cavity is a reservoir of approximately 700 species of microorganisms. Old age necessitates wearing artificial dentures which results in changes in the oral environment and consequently oral flora. Microbes from the oral environment colonize the denture surface to form adherent biofilm which is dependent on the denture characteristics and oral hygiene practices. Aim of the study: To assess presence of different bacterial species in denture wearer. Materials and methods: The study was conducted in the Department of Prosthodontics of the Dental institution. For the study, 50 patients were selected, 25 males and 25 females, who were wearing removable dentures for more than a year and were admitted to the Department of Prosthodontics of the dental institute. 50 individuals with normal dentition and not wearing any dentures were selected as a control group. For the collection of saliva, it was made sure that unstimulated saliva is collected after at least one hour of the last intake of drink or food. The subjects were directed to spit unstimulated saliva into sterile Flacon tubes containing 3 ml thioglycolate broth. Results: We observed that non-oral pathogens were more commonly seen in denture wearers. Gram negative bacilli were seen in 12 denture wearers and 3 non-denture wearers. Similarly, E. coli and E. cloacae are more commonly seen in denture wearers. Conclusion:  From the results of the present study, this can be concluded that patients wearing denture have non-oral pathogenic bacteria in the saliva, which can be a primary cause for increased cases of infections. Thus, improved measures for maintaining oral health should be followed by patients who wear dentures.
Key words: Denture, bacterial species, gram negative bacteria.

Received: 22 April, 2019                            Revised:  28 June 2019                             Accepted: 29 June 2019

Corresponding author: Dr. Sunil Dogra, Senior Lecturer, Department of Prosthodontics, Institute of Dental Sciences, Sehora, Jammu, Jammu and Kashmir, India 
                                                                                 
This article may be cited as: Dogra S, Sharma V, Dhiman T. Assessment of presence of different bacterial species in denture wearer. J Adv Med Dent Scie Res 2019;7(7): 127-130.