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Volume 6 Issue 9 (September, 2018)

Original Articles

Biofilm formation and antibiotic resistance in Methicillin Resistant Staphylococcus aureus among nasal isolated of HIV infected patients in a tertiary care hospital
Manish Kumar Diwakar, Prasanna Gupta, Ankur Goyal

Background: Biofilm production is an important virulence factor of S. aureus. HIV is an established risk factor for MRSA nasal carriage and associated infections. MRSA isolates are more prone to form biofilm which may be a therapeutic emergency in HIV positive patients. Methods: HIV positive patients were taken as cases and Negative as Controls group. Antibiotic resistance was done by Kirby Bauer disc diffusion method. Biofilm formation was detected by Tissue culture plate method, Tube method and Congo red agar method. Result: Out of 96 S. aureus isolates 18 (18.75%) isolates were found to be Methicillin resistant in which 14 (78%) were biofilm producers while 4 (22%) isolates of MRSA were non biofilm producers by TCP method. Among HIV positive case group; 22% & 56% MRSA isolates were strong & moderate biofilm producer while, none of the MRSA isolate was strong biofilm producer among HIV Negative control group isolates. Tissue culture plate method was found 100% specific & 100% sensitive and 100% accurate method as compared to other methods. Chi square test for antibiotic resistance of Biofilm producer MRSA among HIV positive and HIV Negative groups showed significant difference for all the 16 antibiotics enlisted. All the isolates showed 100 % sensitive to Vancomycin, Teicoplanin and Linezolid while resistance against Amikacin, gentamycin and Tetracycline was shown only by biofilm producers. Conclusion: We recommend the TCP method as “Gold Standard” for biofilm detection in routine screening procedures. Keywords: Biofilm, Methicillin resistance, HIV, Nasal carriage, Antibiotic resistance.

 
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