Categories

Volume 6 Issue 5 (May, 2018)

Original Articles

Flourescent Staining in Liquid Based and Conventional Cytology: A Comparative Study
Divya Sharma, Amit K Nayar, Andleeb Manhas, Swati, Aman Sharma, Sadaf Antoo

Background: Cancer is a major public health problem in modern world. The oral cavity witnesses the development of variety of red and white lesions many of which are associated with tobacco use. These lesions often precede the development of frank cancerous lesions known as potentially malignant disorders.The increasing prevalence of Oral cancer and Potentially Malignant Disorders in India has led to an increased need for diagnostic and prognostic markers which are reliable, easy to perform and do not need expensive equipment. Therefore,we felt that there is urgent need for the development of the new technologies in the field of the cytology to identify the most accurate site for biopsy in potentially maligdisorders. Material and methods: A total of 30 Patients with clinically diagnosed Potentially Malignant Disorders was taken from archives of deparment.4 smears were taken from each patient and fixed. The sample was divided into following 2 major groups:GROUP 1 (Conventional cytology) : which are further categorized on basis of staining as:Group1a) Acridine Orange stain,Group 1b) Feulgen Acriflavine stainII)GROUP 2(Liquid based cytology): which were further categorized on the basis of staining as: Group 2a) Acridine Orange stain, Group 2b) Feulgen Acriflavine stain. Results:Among 40 samples of conventional cytology47.50%were found positive and 21 (52.50%) were found negative.out of these positive cases (60.00%) were positive for Acridine orange whereas(35.00%) were positive for Feulgen Acriflavine.Among 40 samples of liquid based cytology, total 13 (32.50%) were found positive and 27 (67.50%) were found negative. Of these 8 (40.00%) were positive for Acridine orange and 5 (25.00%) were positive for Feulgen Acriflavine staining.Therefore, in our study, almost 50 % of theslides made by conventional cytology were positive as compared to only 32% of slide made by liquid based cytology.Out of total 40 samplesboth conventional and liquid based cytology showed clear background for 9 (50.00%) samples each.Out of total 40 samples stained with PAP stain, 19 (47.50%) showed positive uniformity of distribution and 21 (52.50%) showed lack of uniformity of distribution. Among 19 (47.50%) positives, 8 (40.00%) samples were with conventional cytology and 11 (55.00%) were of liquid based cytology. Conclusion: Modified techniques like liquid based cytology have been reported to be useful tool for screening oral premalignant and malignant lesions. We attempted to evaluate the efficacy of this technique using fluorescent staining.In our hands liquid based cytology failed to establish any significance advantages over conventional cytology.Among the fluorescent stains, we found Acridine orange to show better results than Acriflavine Feulgen. It’s lesser cost and ease of use warrants its consideration as an adjective to Exfoliative cytology. Key words: Exfoliative Cytology, fluorescent stains, Oral cancer.

 
Html View | Download PDF | Current Issue