Background: Tuberculosis (TB) is caused by Mycobacterium tuberculosis. It is a chronic bacterial contagious disease that continues to be major public health problem in the world. The present study was conducted for microbiological assessment of patients with tuberculosis in study population. Materials & Methods: The present study was conducted on 520 subjects with suspected tuberculosis. It comprised of 340 males and 180 females. From all subjects sputum was collected and direct smears were prepared, and then each of smear specimens was air dried, fixed, stained with Ziehl-Neelsen (Z-N), and examined under a microscope following standard procedure. Acid fast bacilli (AFB) were detected under microscope and were labeled as AFB positive or negative. Results: Out of 340 males, 120 were found to be AFB +ve and out of 180 females, 90 were found AFB +ve.Age group 10- 20 years had 24 males and 15 females, 20-30 years had 26 males and 27 females, age group 30- 40 years had 45 males and 34 females and >40 years had 25 males and 14 females. The difference was non- significant (P> 0.05).Bacillary load in sputum was paucibacillary (35%) and multibacillary (65%). Conclusion: Myobacterium tuberculosis infection in males was found to be higher than females. Bacillary load in sputum was paucibacillary and multibacillary.
Key words: Acid fast bacilli,Myobacterium tuberculosis, Ziehl-Neelsen