Introduction: Many countries, including the United States, Canada, European Union member states, and others, require that alternatives to animal experimentation for pharmacological research. Hence, attention needs to be given to assess the medicinal value of herbs using in vitro methods. Objective: The present study was carried out to evaluate the anti inflammatory activity of ethanolicextractof Allium cepa peel. Methods: The present research work had carried out on laboratory level assay to avoid the use of different animal models. Hence, ethanolic extract of Allium cepa (EEAC) peels were investigated for anti-inflammatory activity by simple, reliable, less toxic and less time consuming HRBC membrane stabilization method. The presentation of hypo tonicity induced HRBC membrane lysis was taken a measure of anti-inflammatory activity. Their activities were compared with standard drug diclofenac sodium. Results: The results of the study demonstrated that Allium cepa contains active constituents, which possess anti-inflammatory activity which is probably related to the inhibition of prostaglandin synthesis. Conclusion: The result obtained from this study was indicating that ethanolic extract of onion skin possesses significant anti-inflammatory activity and this is a possible rationale for its folkloric use as an anti-inflammatory agent.
Key words: HRBC membrane stabilization, Anti-inflammatory, Diclofenac, Aliumcepa, ,ethanolic extract.