Background: Oral submucous fibrosis (OSMF) is a chronic debilitating disease and a premalignant condition of the oral cavity. Of late, a renewed interest in the role of copper and zinc as a mediator of fibrosis in OSMF has been postulated. Hence; we planned the present study to assess the role of mucosal copper and zinc levels in OSMF patients. Materials & methods: The present study was carried on the patients that were diagnosed histopathologically as Oral Submucous Fibrosis (OSMF). A total of 40 cases of OSMF and 20 cases of without any lesion and habit (normal control) were included in the present study. The preserved tissue samples were weighed initially and an aliquot was prepared by dissolving it in 1ml concentrated nitric acid. This aliquot was then diluted with 2 ml deionized water. For the copper estimation a dilution in the ratio of 1:1 of the solution was used and a ratio of 1:5 for zinc estimation. Estimation of the copper and zinc content was achieved by the colourimetric method. The absorbance of these samples was compared to that of the standard solution provided in the kits at 578 nm in a digital photometer. All the results were analyzed by SPSS software. Results: While comparing mean mucosal copper and zinc levels in between OSMF patients and normal controls, we obtained significant results. Conclusion: Crucial role is played by copper and zinc in the pathogenesis of OSMF.
Key words: Copper, Mucosal, Oral submucous fibrosis, Zinc